Frontiers in Neurophotonics 2008

Download the Frontier in Neurophotonics Summer School 2008 poster & program (3.13MB)

Topics to be covered include:

FRET-FLIM

- Different approaches for FRET: spectral, acceptor photobleaching method and Fluorescence Lifetime Imaging Microscopy (FLIM).
- Applied to the protein-protein interactions and ion fluctuations in different subcellular compartments of a neuron.

Cell tracking in live tissue

- Tracking live cells migration and differentiation in live tissue using different labeling strategies (retroviral infections, single cell electroporation, etc.) and imaging modalities

Live imaging of GFP-tagged protein translocation in cultured neurons

- Time lapse imaging of the dynamic translocation of GFP-tagged intracellular proteins in live neurons.
- Measurements of movement dynamics with FRAP approaches and photo-stimulation of proteins tagged with photo-inducible GFP.

Video-rate wide-field imaging of single molecule dynamics

- Tracking of single membrane receptors on cultured neurons with Quantum dots as fluorescent tags.
- Monitoring of synaptic receptor lateral movement on the membrane in and out of synapses.
- Quantification of receptor mobility, area covered, dwell time inside synapse, etc.

Linear and non-linear video-rate microcopy in live animals

- Animal preparation, optical tool, video-rate hardware, movement correction

CARS Microscopy

- Myelin imaging in nerves and spinal cord using Coherent Raman