Frontiers in Neurophotonics 2009

Download the Frontier in Neurophotonics Summer School 2009 poster (739KB)

Download the Frontier in Neurophotonics Summer School 2009 program (136KB)

Topics to be covered include:

FRET-FLIM

- Different approaches for FRET: spectral, acceptor photobleaching method and Fluorescence Lifetime Imaging Microscopy (FLIM).

- Applied to the protein-protein interactions and ion fluctuations in different subcellular compartments of a neuron.

Cell tracking in live tissue

- Tracking live cells migration and differentiation in live tissue using different labeling strategies (retroviral infections, single cell electroporation, etc.) and imaging modalities

- Two-photon calcium imaging in neuron dendrites in acute hippocampal brain slices

- Imaging Ca²⁺ transients evoked by action potentials and synaptic stimulation

- Quantitative analysis of Ca²⁺ transients: intracellular concentration, fluctuations and endogenous buffering capacity, transients with dual indicators.

Optical mapping of synaptic connectivity between inhibitory interneurons in acute hippocampal slices:

-Two-photon photostimulation of hippocampal interneurons by glutamate uncaging; Mapping synaptic connectivity with two-photon photostimulation

Live imaging of GFP-tagged protein translocation in cultured neurons

- Time lapse imaging of the dynamic translocation of GFP-tagged intracellular proteins in live neurons.
- Measurements of movement dynamics with FRAP approaches and photo-stimulation of proteins tagged with photo-inducible GFP.

Video-rate wide-field imaging of single molecule dynamics

- Tracking of single membrane receptors on cultured neurons with Quantum dots as fluorescent tags.

- Monitoring of synaptic receptor lateral movement on the membrane in and out of synapses.

- Quantification of receptor mobility, area covered, dwell time inside synapse, etc.

Linear and non-linear video-rate microcopy in live animals

- Animal preparation, optical tool, video-rate hardware, movement correction

CARS Microscopy

- Myelin imaging in nerves and spinal cord using Coherent Raman