Frontiers in Neurophotonics 2011

Download the Frontier in Neurophotonics Summer School 2011 poster (557KB)

Topics covered include:

Hands-on sessions (Wednesday 18 to Friday 27):

In vivo activation and monitoring of ChR2 expressing cells with a micro-optrode

The goal of this experience is to activate ChR2 expressing cells in deep tissue in vivo, record the evoked electrophysiological activity and evaluate the input-output curve. Lean more...

3D Stabilized non-linear video-rate microscopy and microglial activation in live animal 

In vivo imaging of the spinal cord in adult animals, for morphological or functional studies, is particularly interesting because it provides rich dynamic information in the context of the intact nervous system as a whole. Learn more...

Two-photon microscopy and optogenetic tools in acute brain slices

Two-photon excitation laser scanning microscopy (2PLSM) in combination with Ca²⁺ imaging and optogenetic tools reveal tremendous details of the dynamic organization of synaptic activity and neuronal circuits in brain slices in vitro. Learn more...

Calcium imaging in neuronal subcompartments using genetically encoded calcium indicators

Calcium is one of the important second messengers in neurons. Studying its dynamics in subcellular compartments is thus important to understand how calcium oscillations can activate opposite signaling pathways in specific locations. Learn more...

Olfactory bulb neurogenesis as a model for studying cell migration and differentiation in the adult tissue

Although the majority of the cells in the nervous system are born during the embryonic and early postnatal period, newborn neurons continue to be generated within at least two areas of the adult mammalian brain. Learn more...

CARS microscopy

Recent advances in laser physics have enabled the development of a new kind of microscopy based on the Raman scattering effect. Coherent anti-Stokes Raman scattering (CARS) is a label-free techniques with a contrast mechanism based on the same vibrational  signatures of molecules seen in Raman spectroscopy but orders on magnitude more sensitive. Learn more...

2011 Summer school program.

The school will be divided into two distinct parts:

During the first week, tutorials and courses will be offered in the morning while afternoons will be entirely dedicated to hands-on experiments in the laboratories. Participants will directly perform the experiments under the supervision of our experts.

In the second part of the school, students will be leading a small research projects on a given topic of their choice. The list of available topics will be published shortly and applicants will be encouraged to list several choices by order of preference.
We will do our best to offer each students the topic of their choice depending on availability and demand.

Wednesday 18

9:00-10:30 Daniel Côté: Basics of lasers and optical imaging
10:30-11:00 Coffee break
11:00-12:30 Ed Ruthazer: Tools for imaging neuronal morphogenesis and synaptogenesis in vivo
12:30-13:30 Lunch
13:30-14:30 Experiment preview
14:30-18 :30 Hands-on Lab experiments
18:30-19:30 Dinner
19:30-22:00 Data analysis

Thursday 19

9:00-10:30 Paul De Koninck : Tracking proteins on the move in neurons
10:30-11:00 Coffee break
11:00-12:30 Mark Niedre: Tomographic Imaging with Diffuse Light: Molecular Contrast at Preclinical and Clinical Scales
12:30-13:30 Lunch
13:30-14:30 Experiment preview
14:30-18 :30 Hands-on Lab experiments
18:30-19:30 Dinner
19:30-22:00 Data analysis

Friday 20

9:00-10:30 Daniel Côté: Multimodal cellular imaging in vivo
10:30-11:00 Coffee break
11:00:12:30 Kurt Haas: Developmental neuronal plasticity
12:30-13:30 Lunch
13:30-14:30 Experiment preview
14:30-18 :30 Hands-on Lab experiments
18:30-19:30 Dinner
19:30-22:00 Data analysis

Saturday 21

9:00-10:30 Tim Ford: Optically Sectioned Widefield Fluorescence Imaging with HiLo
10:30-11:00 Coffee break
11:00-12:30 Lisa Topolnik: Functional calcium imaging: principles, advantages and pitfalls
12:30-13:30 Lunch
13:30-14:30 Experiment preview
14:30-18 :30 Hands-on Lab experiments
18:30-19:30 Dinner
19:30-22:00 Data analysis

Sunday 22 - Free Time

Monday 23

9:00-10:30 Paul Selvin: High resolution microscopy based on single molecule imaging/photobleaching
10:30-11:00 Coffee break
11:00-12:30 Thomas Blanpied: Using PALM for live-cell investigation of intracellular protein dynamics
12:30-13:30 Lunch
13:30-14:30 Experiment preview
14:30-18 :30 Hands-on Lab experiments
18:30-19:30 Dinner
19:30-22:00 Data analysis

Tuesday 24

9:00-10:30 Ryohei Yasuda: Fluorescence lifetime imaging microscopy (TPFLIM) and synaptic plasticity
10:30-11:00 Coffee break
11:00-12:30 Projects
12:30-13:30 Lunch
13:30-18 :30 Projects
18:30-19:30 Dinner
 

Wednesday 25

9:00-10:30 Antoine Adamantidis: Experimental strategies for in vitro and in vivo optogenetics
10:30-11:00 Coffee break
11:00-12:30 Projects
12:30-13:30 Lunch
13:30-18 :30 Projects
18:30-19:30 Coherent’s Group dinner
 

Thursday 26

9:00-10:30 Robert Campbell: Genetically encoded fluorescent labels
10:30-11:00 Coffee break
11:00-12:30 Projects
12:30-13:30 Lunch
13:30-18 :30 Projects
18:30-19:30 Dinner
 

Friday 27

9:00-10:30 Tim Murphy: Imaging and optogenetic tools for studying cortical circuit structure and function in healthy brain and after stroke
10:30-11:00 Coffee break
11:00-12:30 Projects
12:30-13:30 Lunch
13:30-18 :30 Students presentations (until 15h30)